Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
نویسندگان
چکیده
منابع مشابه
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays.
The reverse transcription polymerase chain reaction (RT-PCR) is the most sensitive method for the detection of low-abundance mRNA, often obtained from limited tissue samples. However, it is a complex technique, there are substantial problems associated with its true sensitivity, reproducibility and specificity and, as a quantitative method, it suffers from the problems inherent in PCR. The rece...
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Polymerase chain reaction (PCR)-based assays can target either DNA (the genome) or RNA (the transcriptome). Targeting the genome generates robust data that are informative and, most importantly, generally applicable. This is because the information contained within the genome is context-independent; i.e., generally, every normal cell contains the same DNA sequence--the same mutations and polymo...
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ژورنال
عنوان ژورنال: Journal of Molecular Endocrinology
سال: 2000
ISSN: 0952-5041,1479-6813
DOI: 10.1677/jme.0.0250169